Bleomycin, one antibiotic drug, produced by a strain of Streptomyces verticillus is used effectively in the treatment of various cancers. It is well known that bleomycin acts on the cancer cell by bindng to nuclear DNA, causing single-strand
scission
and in turn inhibiting DNA synthesis. Although bleomycin is very beneficial to cancer patients, it may damage to normal cells. The author has undertaken this study to purses the morphological changes of hair follicles caused by bleomycin
injection.
Healthy male mice of DDY strain weighing about 20 gm were used in this experiment. Animals of the experimental group were intraperitoneally administrated with 15mg/kg bleomycin diluted in 0.2ml distilled water for injection by bid for 7 days. The
experimental animals were sacrified at 1, 3 days, and 1, 2 weeks after administration of blecomycin and specimens, skin of abdomen, were resected. Some specimens were fixed 10% formalin for 24 hours and stained with hematoxylin and eosin, the
otehrs
were prefixed I 2% glutaraldehyde-2.5% paraformaldehyde solution at 4℃ during 2-4 hours and postifixed in 1% osmium tetroxide solution. Ultrathin sections were mode and double stained with uranyl acetate and lead citrate. These preparations were
observed with light microscope and electron microscope (Hitachi H-600), respectively.
@ES the results were as follows:
@EN 1. The umber and length of the hair follicles were decreased and number of matrix cells were also reduced in skin in 1 day, 3 day and 1 week group after administration of bleomycin. In 2 week group, hair follicles were similar to those of the
normal
control group.
2. swollen mitochondria, mitochondria with indistinct double membranes and mitochondrial cristae, and degenerated mitochondria with horse-shoe shape were recognized in 1 day, 3 day and 1 week group after bleomycin administration. In 2 week
group,
swollen mitocondria and mitochondria with disorganized mitochondrial cristae and double membranes were observed.
3. The amounts of free ribosomes were markedly reduced in 1 day group after administration of bleomycin and slightly decreased in 3 day and 1 week group. In 2 week group, distribution of free ribosomes were similar to that of the normal control
group.
4. The cisternae of the rough endoplasmic reticulum with detachment of membrane-bound ribosomes were slightly dilated in 1 day, 3 day groups after administration of bleomycin. In 2 week group, rough endoplasmic reticulum were similar to that of
the
normal control group
5. A number of autophagic vacuoles were observed in 1 day, 3 day group after administration of bleomycin and whorled membraneous body was seen in 1 week group
Consequently, it is suggested that bleomycin would induced degenerative changes on the matrix cells of the hair follicle in mouse skin, but these damage are recovered as time goes by.
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